Regarding the MagnaLink™ Streptavidin beads, can the amount of biotin bound to the bead be quantitated using a fluorescent tag? Does the bead quench the fluorescence in any way?
A:
The covalent bond used to immobilize streptavidin on the beads is not affected by the presence of this detergent. The only detergent that we definitely know destroys all biotin binding...
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