After purifying the chromatin fragments from the formaldehyde-fixed cells, the team immunoprecipitated the DNA-protein complexes using an anti-H4 antibody. Following reversal of the crosslinks, they ...

Varying levels and types of histone modifications have been shown to correlate with levels of chromatin activation. For example, one group of researchers used antibody-based immunoprecipitation ...

chromatin regulatory, and metabolism proteins. We continuously work together with BCM investigators to build RPPA assays for new protein pathways of interest and to validate the required antibodies.

using antibodies and beads to pull down the modified histone of interest and its associated DNA, a technique known as chromatin immunoprecipitation, or ChIP. Next, the investigators queried the ...